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Spectroscopy
Volume 25 (2011), Issue 5, Pages 243-250
http://dx.doi.org/10.3233/SPE-2011-0509

Application of FTIR spectroscopy coupled with chemometrics for authentication of Nigella sativa seed oil

A. F. Nurrulhidayah,1 Y. B. Che Man,1,2,3,6 H. A. Al-Kahtani,3 and A. Rohman4,5

1Halal Products Research Institute, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
2Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
3College of Food and Agriculture Science, Department of Food Science and Nutrition, King Saud University, Riyadh, Saudi Arabia
4Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia
5Halal Research Group, Gadjah Mada University, Yogyakarta, Indonesia
6Halal Products Research Institute, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study is intended to analyze the presence of grape seed oil (GSO) in Nigella sativa L. seed oil (NSO) using Fourier transform infrared (FTIR) spectroscopy and gas chromatography (GC). FTIR spectroscopy coupled with multivariate calibration of partial least square can quantify the levels of GSO in NSO at wavelength number of 1114–1074, 1734–1382 and 3005–3030 cm–1. The coefficient of correlation (R2) obtained for the relationship between actual (x-axis) and FTIR predicted (y-axis) values are 0.981. The errors in cross validation and in prediction are 2.34% (v/v) and 2.37% (v/v), respectively.