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Volume 26, Issue 4-5, Pages 289-296

DNA binding and photo-induced DNA cleavage activity of Elsinochrome A in visible light

Fei Ma,1 Jing Tang,1 Chao Yang,1 Yu-Ying Feng,1 Shao-Hua Wei,1 Jian Shen,1 Jia-Hong Zhou,1,2 and Lin Zhou1

1Jiangsu Key Laboratory Biofunctional Materials, Nanjing Normal University, Nanjing, China
2Jiangsu Key Laboratory Biofunctional Materials, Nanjing Normal University, Nanjing-210097, China

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The interaction of Elsinochrome A (EA) with calf thymus DNA (CT-DNA) has been investigated by UV-vis spectra and fluorescence spectra. The results show that EA can bind with CT-DNA and binding sites are destroyed after irradiation by visible light, which indicates that EA is a promising candidate for photodynamic therapy. In addition, the binding mechanism is studied using fluorescence quenching test and ethidium bromide (EB) replace assay experiments. The results suggest that EA and CT-DNA are binding with a strong force and the major binding mode of EA with DNA could be the electrostatic binding.