Determination of Collagen Fibers Arrangement in Bone Tissue by Using Transformations of Raman Spectra Maps
Figure 3
The contrast images obtained by using transformations of Raman spectra maps. The contrast images with difference values between /amide I ratio values obtained for the HH polarization and H polarization [HH−H] (a), for the VV polarization and V polarization [VV−V] (b). The contrast images with the total values [(VV−V) + (HH−H)], which is the sum of [VV−V] values and [HH−H] values from the first transformation (c). Arrows indicate collagen fibers arrangement on surface of trabecula, and the color bar displays the maximum value for each image.
