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Spectroscopy: An International Journal
Volume 27 (2012), Article ID 540490, 5 pages

Raman Spectroscopy of Bacterial Species and Strains Cultivated under Reproducible Conditions

1Department of Physics, Swansea University, Swansea SA2 8PP, UK
2Department of Medical Microbiology and Infectious Diseases, Institute of Life Science, Swansea University, Swansea SA2 8PP, UK

Copyright © 2012 Jamal F. M. Almarashi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Rapid and reproducible discrimination between bacterial pathogens is a clear goal in microbiological laboratories when processing infected clinical samples. In this study Raman spectra were taken from at least 30 colonies of four strains of bacteria including Staphylococcus epidermidis (1457 and 9142) and Escherichia coli (K12 and Top 10) using the Renishaw in Via Raman microscope system. Analysis based on principal components suggests that even strain differentiation (e.g., 1457 versus 9142 or K12 versus Top10) is possible.