Spectroscopic Determination of pKa Constants of MADS Box Segments

Figure 1

Fluorescence pH dependency of the MADS box segment Ac-IMKKAYEL-amid (a), determined fractions (b), normalized emission spectra of the two components—normalization factor 𝑁_𝐴=0.003, 𝑁_𝐵=0.201 (c), and factor analysis results (d).