A New Method for D₂O/H₂O Exchange in Infrared Spectroscopy of Proteins

Figure 1

(a) FTIR spectra recorded in a RC film equilibrated at 𝑟=76% (blue), dehydrated at 𝑟=11% (black), and rehydrated at 𝑟=76% in the presence of D₂O (red). (b) Enlargement of the 5500–4700 cm⁻¹ spectral region, showing the (𝜈₂+𝜈₃) combination band of water at 5150 cm⁻¹.

(a)

(b)