Development and Validation of UV-Visible Spectrophotometric Baseline Manipulation Method for Simultaneous Quantitation of Tenofovir Disoproxil Fumarate and Emtricitabine in Pharmaceutical Dosage Form

Table 1

Optical characteristics of the method and result of formulation analysis, recovery study, and method sensitivity.

Parameter	Analyte
	TE	EM
λ max (nm)	261	289.9
Range (μg/mL)	4–20	6–30
Regression equation 𝑌=𝑚𝑥+𝑐
Slope (m)	0.05974	0.022127
Intercept (𝑐)	−0.23012	−0.01772
Correlation coefficient	0.999	0.999
Limit of quantitation (LOQ)	1.58	4.5
Limit of detection (LOD)	0.55	1.5
Formulation analysis (% assay, % RSD)
F I	99.75, 0.84	99.35, 1.01
F II	101.2, 1.23	100.2, 0.97
Recovery study at the level (% R, % RSD)
50%	100.21, 0.54	99.5, 0.45
100%	100.4, 0.94	101.4, 0.75
150%	99.45, 0.54	99.78, 0.69