Development and Validation of UV-Visible Spectrophotometric Baseline Manipulation Method for Simultaneous Quantitation of Tenofovir Disoproxil Fumarate and Emtricitabine in Pharmaceutical Dosage Form

Table 5

Robustness study for TE and EM.

Factor	Level	Mean % assay, % RSD
		TE	EM
Shaking time (±2 min)	10	99.2, 0.97	99.34, 0.87
	6	98.18, 0.25	98.42, 1.23
Measurement wavelength (±1 nm)	260 (EM), 288.9 (TE)	99.45, 0.43	99.43, 0.54
	262 (EM), 290.9 (TE)	99.08, 0.48	99.65, 0.64
Concentration of TE in blank (±1 μg/mL)	21	99.09, 0.72	100.45, 1.34
	19	100.3, 0.59	99.78, 0.45