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Journal of Spectroscopy
Volume 2013 (2013), Article ID 538608, 7 pages
Research Article

Spectral Characterization of Fungal Metabolites in Aqueous Medium with Humus Substances

1Faculty of Physics, Lomonosov Moscow State University, Moscow 119991, Russia
2Faculty of Soil Science, Lomonosov Moscow State University, Moscow 119991, Russia
3Institute of Ecology and Evolution, Russian Academy of Sciences, 33 Leninskiy Prospesct, Moscow 119071, Russia

Received 30 June 2012; Accepted 6 September 2012

Academic Editor: Maciej Sitarz

Copyright © 2013 D. A. Khundzhua et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The work is targeted to confirm participation of microscopic fungi in transformation of humus substances in aquatic environments. The research is focused on the spectroscopic study of the collection of fungal strains with different pigmentation of mycelium. Spectral properties of fungal metabolites were measured and compared to that of natural aquatic nonliving organic matter and commercial humus substances in aqueous solutions. The experiments revealed that the effect of microscopic fungi growing in the culture medium with added humate appeared as changes in the humic-type fluorescence: its characteristics became more similar to that of nonliving organic matter in natural waters than to original humate preparation. The experiments demonstrated degradation of coal-originated humate due to microbial activity into compounds of smaller molecular size and increased heterogeneity. We resume that transformation of humus substances by fungal cultures can be monitored and characterized using spectral measurements.