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Journal of Spectroscopy
Volume 2013, Article ID 810247, 6 pages
Research Article

Study on Fracture Healing with Small-Splint-Fixation Therapy by Near-Infrared Raman Spectroscopy

1College of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China
2Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou 350007, China

Received 2 May 2012; Revised 22 July 2012; Accepted 26 July 2012

Academic Editor: Kong-Thon Tsen

Copyright © 2013 Hao Huang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In this study, near-infrared (NIR) Raman spectroscopy was explored to assess the incorporation of calcium hydroxyapatite (CHA ~960 c m 1 ) and other biochemical substances during the recovery of rabbits with complete radial fractures treated with or without small splints. 24 rabbits were randomy divided into two groups, one treated with small-splint-fixation therapy and the other without any intervention. The rabbits were sacrificed at 7, 15, 23, and 30 days after surgery, and the surface layers of the calluses in the fracture healing site from control and treated groups were routinely prepared for Raman spectroscopy. The prominent Raman bands were observed, including minerals at 430, 590, 960, 1003, and 1071 c m 1 , protein at 856, 876, 1246, and 1667 c m 1 , and lipid at 1767 c m 1 . The carbonate-to-phosphate ratio (CO3 to υ1 PO4) and the mineral-to-matrix ratio (υ1 PO4 to amide I) were calculated from these normalized Raman bands. Comparison of the υ1 PO4-to-amide I ratio for the control group with that of the treated group probably indicated that the small-splint-fixation therapy could be useful for the gradual mineralization of the collagen matrix during fracture healing.