Development and Validation of Spectrophotometric Methods for the Determination of Rasagiline in Pharmaceutical Preparations

Table 1

Results of analytical parameters for proposed methods.

	Method A	Method B	Method C
Linearity range^a [μg mL^−1]	25–300	25–350	50–500
Molar absorptivity [l mol^−1 cm^−1]	3.46 × 10^3	2.31 × 10^3	1.6 × 10^3
Sandel’s sensitivity [μg cm^−2 per 0.001 absorbance unit]	5.56 × 10^−2	7.41 × 10^−2	1.02 × 10^−1
Precision
Intraday^b, RSD %	0.75	0.82	0.56
Interday^c, RSD %	1.02	1.15	1.27
Regression equation^d
Slope ± SD	0.0028 ± 0.00006	0.0024 ± 0.00002	0.0015 ± 0.00003
Intercept ± SD	0.0777 ± 0.00011	0.0263 ± 0.00024	0.0381 ± 0.00005
Correlation coefficient, 𝑟	0.9996	0.9996	0.9983
LOD [μg mL^−1]	0.122	0.279	0.583
LOQ [μg mL^−1]	0.407	0.932	1.94

aAverage of six determinations.
^b𝑛=5 corresponding to replicate analysis for each level.
^cResults of five different days.
^d𝐴=𝑎+𝑏𝐶 [𝐶 is the concentration of drug in μg mL⁻¹ and 𝐴 is the absorbance at 𝜆_max].