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Journal of Spectroscopy
Volume 2013, Article ID 971914, 6 pages
Research Article

Metabolic Alterations of the Zebrafish Brain after Acute Alcohol Treatment by 1H Nuclear Magnetic Resonance Spectroscopy

1Department of Radiology, UT Southwestern Medical Center, Dallas, TX, USA
2Department of Biomedical Engineering, Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea
3Department of Molecular Biology, College of Medicine, The Catholic University of Korea, Republic of Korea
4Environment & Metabolomics Research Team, Seoul Center, Korea Basic Science Institute, Seoul, Republic of Korea
5Department of Radiology, Cheongju University, Cheongju, Chungbuk, Republic of Korea

Received 22 February 2013; Accepted 8 May 2013

Academic Editor: Tomasz Ruman

Copyright © 2013 Dong-Cheol Woo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The purpose of this study is to investigate the metabolic alterations associated with acute alcohol treatment in zebrafish by 1H nuclear magnetic resonance spectroscopy (NMRS). The brain metabolism of zebrafish was investigated after acute alcohol treatment (one-hour long exposure of adult fish to 0.00%, 0.25%, 0.50%, or 1.00% ethyl alcohol) with whole brain extraction. The results of this study showed that glutamate (Glu) was significantly decreased, scyllo-inositol (sIns) showed a small apparent increase only in the highest acute treatment dose group, and myoinositol (mIns) showed a significant decrease. [Glu]/[tCr] and [mIns]/[tCr] levels were significantly reduced regardless of the alcohol dose, and [sIns]/[tCr] was increased in the highest alcohol treatment dose group. The present NMR study revealed that specific metabolites, such as Glu and mIns, were substantially decreased in case of acute alcohol exposed zebrafish brain.