Spectroscopic Study of Cytosine Methylation Effect on Thermodynamics of DNA Duplex Containing CpG Motif

Figure 3

Example ¹H NMR spectra of OctAT in the region of nonexchangeable base hydrogen signals at temperatures when duplexes are formed (bottom), in the melting region (middle), and when only separated strands are present (top). Black lines show the experimental data and red ones the results of the fits (details in the text). The assignments of the NMR lines used for the melting temperature determination are denoted as the following: the type of the nucleobase, its position in the sequence from the 5′ side, and position of the hydrogen in respect of the purine or pyrimidine ring.