Spectroscopic Study of Cytosine Methylation Effect on Thermodynamics of DNA Duplex Containing CpG Motif

Table 1

The melting temperatures determined by particular spectroscopic techniques.

Sample	UV absorption		^1H NMR		Raman scattering
	(M)	(°C)	(mM)	(°C)	(mM)	(°C)
OctAT	11.7 ± 0.2	36.4 ± 0.5	1.17 ± 0.02	50.7 ± 0.6	2.7 ± 0.1	54.6 ± 0.8
mOctAT	9.6 ± 0.2	38.2 ± 0.6	1.12 ± 0.02	52.4 ± 0.5	2.2 ± 0.1	54.4 ± 0.9
OctTT	12.0 ± 0.2	26.9 ± 0.6	1.06 ± 0.02	45.1 ± 1.0	2.8 ± 0.1	49.8 ± 0.8
mOctTT	12.6 ± 0.2	27.1 ± 0.6	0.92 ± 0.02	45.7 ± 1.0	2.9 ± 0.1	52.6 ± 0.9
OctAA	9.8 ± 0.2	35.6 ± 0.5	1.10 ± 0.02	51.0 ± 0.6	2.3 ± 0.1	53.3 ± 1.0
mOctAA	11.8 ± 0.2	37.7 ± 0.6	1.01 ± 0.02	51.9 ± 0.6	2.7 ± 0.1	57.0 ± 1.2