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Journal of Spectroscopy
Volume 2016, Article ID 1596845, 7 pages
Research Article

Discrimination of Nasopharyngeal Carcinoma from Noncancerous Ex Vivo Tissue Using Reflectance Spectroscopy

1Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China
2Fujian Metrology Institute, Fuzhou 350003, China
3College of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China
4Affiliated Fuzhou First Hospital of Fujian Medical University, Fuzhou 350009, China

Received 2 January 2016; Revised 16 March 2016; Accepted 28 March 2016

Academic Editor: Rickson C. Mesquita

Copyright © 2016 Zhihong Xu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Reflectance spectroscopy is a low-cost, nondestructive, and noninvasive method for detection of neoplastic lesions of mucosal tissue. This study aims to evaluate the capability of reflectance spectroscopy system under white light (400–700 nm) with a multivariate statistical analysis for distinguishing nasopharyngeal carcinoma (NPC) from nasopharyngeal benign ex vivo tissues. High quality reflectance spectra were acquired from nasopharyngeal ex vivo tissues belonging to 18 noncancerous and 19 cancerous subjects, and the combination of principal component analysis-linear discriminant analysis (PCA-LDA) along with leave-one-spectrum-out cross-validation (LOOCV) diagnostic algorithm was subsequently employed to classify different types of tissue group, achieving a diagnostic sensitivity of 73.7% and a specificity of 72.2%. Furthermore, in order to distinguish NPC from nasopharyngeal benign ex vivo tissues based on reflectance spectra simply, spectral intensity ratios of oxyhemoglobin (540/576) were used as an indicator of the carcinogenesis associated transformation in the hemoglobin oxygenation. This tentative work demonstrated the potential of reflectance spectroscopy for NPC detection using ex vivo tissue and has significant experimental and clinical value for further in vivo NPC detection in the future.