Simultaneous Determination of Four Compounds in a Nelumbo nucifera Seed Embryo by HPLC-DAD

Table 1

Calibration curves, LOD, and LOQ of four compounds in N. nucifera embryos. Neferine (1), 1,2,3,4-tetrahydro-1-[(4-hydroxyphenyl) methyl]-2-methyl-7-isoquinolinol (2), 1-hydroxy-2-methylpropene (3), and 3-(prop-1-enyl)benzene-1,2,4,5-tetrol (4).

Compounds	Regression equation	R^2 ()	LOD (μg/mL)	LOQ (μg/mL)
1		0.962	1.3120	3.9758
2		0.999	4.3690	13.2394
3		1	2.2691	6.8759
4		1	1.8396	5.5747