Application of Near-Infrared Spectroscopy to Quantitatively Determine Relative Content of Puccnia striiformis f. sp. tritici DNA in Wheat Leaves in Incubation Period

Table 1

Duplex TaqMan real-time PCR primers and probes used in this study.

Primer/probe	NCBI accession number	Sequence of primers and corresponding probes (5′-3′)	Amplified fragment length (bp)
TAG2315F	AF280605.1	CAGAAAGCGAGTGGAAAGATGAAAG	181
TAG2473R		GCAAGGAGGACAAAGATGAGGAA
TAG-Pr1		HEX-CAAGCATCAAAGGCAAGCAAGCAGTAGT-BHQ1
Pst-F	GU382673.1	AACCCTCTCATTAAATAATTTTG	102
Pst-R		CCAACTTATAGAAAAGTGACTTA
Pst-P		FAM-ATTACAGCAGCACTCAACATCCATT-BHQ1