Combined Raman Spectroscopy and Digital Holographic Microscopy for Sperm Cell Quality Analysis

Figure 3

(a) Pseudo-3D representation of the phase map of the investigated spermatozoon obtained by digital holography microscopy. The arrow indicates the observed protuberance in the region connecting the tail to the head of the spermatozoon. (b) Phase intensity profile of the spermatozoon along the line DD′ stressed in (c) [7].

(a)

(b)

(c)