Combined Raman Spectroscopy and Digital Holographic Microscopy for Sperm Cell Quality Analysis

Figure 8

(a) Phase map of the sperm cell region reconstructed at the focus plane; the phase profile along the lines BB′ (b–d) and AA′ (f–h) and the Raman spectra in the point () are monitored during the laser exposition. (e) 3D representation of the phase map of the investigated spermatozoon obtained by digital holography microscopy [8].

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)