Journal of Spectroscopy / 2018 / Article / Tab 2

Research Article

4-Hydroxycoumarin Derivative: N-(diphenylmethyl)-2-[(2-oxo-2H-chromen-4-yl)oxy]acetamide Interaction with Human Serum Albumin

Table 2

Fluorescence lifetime profiles of HSA and increasing concentration of coumarin derivatives at different excitation wavelengths.

Wavelength (nm)α1 ± (error × 10−3)α2 ± (error × 10−3)α3 ± (error × 10−3)τ1 (ns)τ2 (ns)τ3 (ns) (ns)χ2

Fluorescence decay-fitting parameters for free HSA
2800.00098 ± 0.0120.74 ± 5.130.00015 ± 0.022.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0061.130.5
2900.017 ± 0.0240.61 ± 5.600.00106 ± 0.052.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0062.551.03
3000.037 ± 0.0330.29 ± 5.340.0024 ± 0.072.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0062.841.42
3100.037 ± 0.0360.21 ± 5.130.0047 ± 0.0862.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0063.401.19
3200.037 ± 0.0420.15 ± 5.310.010 ± 0.0132.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0064.241.12
3300.035 ± 0.0460.093 ± 5.340.016 ± 0.0132.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0064.891.09
3400.032 ± 0.0480.042 ± 5.240.020 ± 0.0142.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0065.231.00
3500.027 ± 0.050.027 ± 5.230.024 ± 0.0152.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0065.561.00
3600.026 ± 0.0510.00054 ± 5.00.026 ± 0.0162.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0065.691.05
3700.024 ± 0.0500.0018 ± 5.00.027 ± 0.0162.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0065.790.96
3800.023 ± 0.0520.0061 ± 5.10.029 ± 0.0162.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0065.871.00
3900.022 ± 0.0510.0031 ± 5.10.028 ± 0.0162.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0065.910.91
4000.022 ± 0.0510.016 ± 5.00.029 ± 0.0162.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0065.901.00
4100.024 ± 0.0510.007 ± 5.00.027 ± 0.0162.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0065.811.00
4200.028 ± 0.0510.035 ± 5.00.026 ± 0.0162.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0065.661.00
4300.029 ± 0.0530.029 ± 4.90.024 ± 0.0152.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0065.531.00
4400.030 ± 0.0500.030 ± 4.90.023 ± 0.0152.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0065.501.00
4500.031 ± 0.0500.038 ± 4.90.023 ± 0.0152.12 ± 0.0080.0097 ± 0.0026.82 ± 0.0065.470.99

Fluorescence decay-fitting parameters for the HSA-coumarin derivative complex
2800.0015 ± 0.0110.00004 ± 0.00130.815 ± 5.51.9 ± 0.00826.8 ± 0.00650.009 ± 0.0020.650.34
2900.0051 ± 0.0160.00021 ± 0.00210.806 ± 5.91.9 ± 0.00826.8 ± 0.00650.009 ± 0.0021.550.73
3000.029 ± 0.0300.0015 ± 0.00540.499 ± 6.01.9 ± 0.00826.8 ± 0.00650.009 ± 0.0022.511.07
3100.037 ± 0.0370.0004 ± 0.00730.374 ± 6.11.9 ± 0.00826.8 ± 0.00650.009 ± 0.0023.171.16
3200.038 ± 0.0420.0008 ± 0.00960.20 ± 5.91.9 ± 0.00826.8 ± 0.00650.009 ± 0.0024.031.11
3300.041 ± 0.0490.015 ± 0.0120.095 ± 5.91.9 ± 0.00826.8 ± 0.00650.009 ± 0.0024.691.07
3400.037 ± 0.0500.019 ± 0.0130.025 ± 5.61.9 ± 0.00826.8 ± 0.00650.009 ± 0.0025.110.98
3500.035 ± 0.0530.024 ± 0.0140.012 ± 5.61.9 ± 0.00826.8 ± 0.00650.009 ± 0.0025.411.01
3600.030 ± 0.0530.025 ± 0.0140.020 ± 5.41.9 ± 0.00826.8 ± 0.00650.009 ± 0.0025.590.97
3700.029 ± 0.0550.029 ± 0.0150.035 ± 5.51.9 ± 0.00826.8 ± 0.00650.009 ± 0.0025.731.09
3800.026 ± 0.0540.029 ± 0.0150.049 ± 5.31.9 ± 0.00826.8 ± 0.00650.009 ± 0.0025.801.03
3900.026 ± 0.0550.030 ± 0.0160.072 ± 5.21.9 ± 0.00826.8 ± 0.00650.009 ± 0.0025.830.98
4000.024 ± 0.0540.029 ± 0.0160.075 ± 5.01.9 ± 0.00826.8 ± 0.00650.009 ± 0.0025.891.13
4100.027 ± 0.0550.031 ± 0.0160.11 ± 5.01.9 ± 0.00826.8 ± 0.00650.009 ± 0.0025.851.06
4200.028 ± 0.0540.028 ± 0.0160.15 ± 4.71.9 ± 0.00826.8 ± 0.00650.009 ± 0.0025.761.26
4300.030 ± 0.0540.027 ± 0.0160.17 ± 4.51.9 ± 0.00826.8 ± 0.00650.009 ± 0.0025.671.15
4400.033 ± 0.0540.026 ± 0.0160.19 ± 4.41.9 ± 0.00826.8 ± 0.00650.009 ± 0.0025.501.17
4500.032 ± 0.0530.025 ± 0.0150.21 ± 4.21.9 ± 0.00826.8 ± 0.00650.009 ± 0.0025.531.04

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