Evaluation of Biophysical Interaction between Newly Synthesized Pyrazoline Pyridazine Derivative and Bovine Serum Albumin by Spectroscopic and Molecular Docking Studies

Figure 5

(a) UV absorbance spectra of BSA (1.5 μM) in presence of 5d concentrations (0–18 μM) at 205 nm and 280 nm. (b) The absorption spectrum of BSA (1.5 μM) and 5d (13.5 μM) alone and the spectra of their BSA-5d complex and absorbance of (BSA-5d complex-5d). (c) Plot of the absorbance of the mixture, at 205 and 280 nm, versus the concentration of 5d. (d) Excitation spectra of BSA (1.5 μM) in presence of 5d (0–18 μM).

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