Evaluation of Biophysical Interaction between Newly Synthesized Pyrazoline Pyridazine Derivative and Bovine Serum Albumin by Spectroscopic and Molecular Docking Studies

Table 2

BSA-5d system: binding parameters and thermodynamic parameters.

T (K)	Kb ± SD	n	∆G° ± SD (kJ·mol^−1)	∆H° ± SD (kJ·mol^−1)	∆S° ± SD (J·mol^−1·K^−1)
298	(5.12 ± 0.39) × 10^4	0.999 ± 0.009	−26.83 ± 0.22	78.33 ± 5.86	352.92 ± 18.88
303	(8.31 ± 0.66) × 10^4	1.065 ± 0.007	−28.59 ± 0.13
310	(1.73 ± 0.29) × 10^5	1.155 ± 0.002	−31.07 ± 0.01