Table of Contents Author Guidelines Submit a Manuscript
Journal of Spectroscopy
Volume 2019, Article ID 8083207, 8 pages
https://doi.org/10.1155/2019/8083207
Research Article

Change in the Microviscosity of Erythrocyte Membranes and Proteins in Blood Plasma after Graphene Oxide Addition: The ESR Spectroscopy Study

1B. Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, Kharkiv, Ukraine
2O. Chuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine
3Institute for Single Crystals, National Academy of Sciences of Ukraine, Kharkiv, Ukraine
4Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkiv, Ukraine

Correspondence should be addressed to V. A. Karachevtsev; au.vokrahk.tli@vestvehcarak

Received 7 September 2018; Accepted 19 December 2018; Published 1 April 2019

Academic Editor: Rizwan Hasan Khan

Copyright © 2019 V. A. Karachevtsev et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The change in the microviscosity of erythrocyte membranes and the proteins in blood plasma after graphene oxide addition is studied by the ESR spectroscopy exploiting two spin probes with different lipophilic components in the structures. Experiments with charged spin probe 2 embedded into the erythrocyte membrane showed that the introduction of graphene oxide in small concentrations (∼70 μg/ml) into a suspension of erythrocytes did not lead to significant changes in the microviscosity of their membranes. Correlation times of hydrophobic spin probe 1 adsorbed to hydrophobic pockets of plasma proteins demonstrate a gradual slowdown at the graphene oxide injection into blood plasma that indicates a small deformation of the hydrophobic cavity of protein at the adsorption. However, this protein binding with graphene oxide does not cause the displacement of the spin probe from their hydrophobic cavities, which is evidence about small changes in the protein secondary structure. The obtained results indicate the insignificant cytotoxicity effect of small concentrations of graphene oxide for erythrocytes and blood plasma.