Jak2-Independent Activation of Stat3 by Intracellular Angiotensin II in Human Mesangial Cells

Figure 4

Effect of intracellular Ang II on Stat3 protein expression. (a) A sample picture of Western blot of total cell lysates from mesangial cells incubated with exogenous Ang II (NG + ex-Ang II) or transfected with Ang II (NG + t-Ang II) for 24 h showing Stat3 protein band (92 kDa) and b-actin (42 kDa) (loading control). (b) Densitometry analysis revealed a significant increase in Stat3 protein in cells treated with exogenous Ang II (NG + ex-Ang II) which was blocked by candesartan (C). In Ang II transfected cells (NG + t-Ang II), Stat3 protein expression increased significantly similar to that observed in cells treated with exogenous Ang II. Data are presented as mean ± SEM (𝑛=5). ^∗𝑃<0.05 versus NG; 𝑃̂<0.05 versus NG + ex-Ang II.

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