Individual Rac GTPases Mediate Aspects of Prostate Cancer Cell and Bone Marrow Endothelial Cell Interactions
Bone marrow endothelial cells react to PCa cell binding. (a, b) are measurements of the dynamic elastic modulus or elasticity of PC-3 and BMECs in contact with one another. Elasticity is given as the Young’s modulus and is a ratio of cell stress and strain and is measured in Pascals. BMECs were grown as a monolayer and control (siScr) or siRac1 expressing PC-3 cells were allowed to bind to the BMEC monolayer, and the elasticity of the PC-3 cells (a) and the BMECs (b) was measured by AFM. Data are the result of over 10,000 data points and represented as mean ± S.D. with significance being *. (c) are measurements of transendothelial electrical resistance (TEER). BMECs were grown on a monolayer, PC-3 cells were added to the monolayer, and the electrical resistance was measured every 10 min up to 1 h (i) and the final measurement at 24 h (ii).
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