Individual Rac GTPases Mediate Aspects of Prostate Cancer Cell and Bone Marrow Endothelial Cell Interactions
Figure 4
Bone marrow endothelial cells react to PCa cell binding. (a, b) are measurements of the dynamic elastic modulus or elasticity of PC-3 and BMECs in contact with one another. Elasticity is given as the Young’s modulus and is a ratio of cell stress and strain and is measured in Pascals. BMECs were grown as a monolayer and control (siScr) or siRac1 expressing PC-3 cells were allowed to bind to the BMEC monolayer, and the elasticity of the PC-3 cells (a) and the BMECs (b) was measured by AFM. Data are the result of over 10,000 data points and represented as mean ± S.D. with significance being *. (c) are measurements of transendothelial electrical resistance (TEER). BMECs were grown on a monolayer, PC-3 cells were added to the monolayer, and the electrical resistance was measured every 10 min up to 1 h (i) and the final measurement at 24 h (ii).
