Figure 2: Alignment of CISH molecules from selected tetrapod and fish species. The multiple alignment was produced using the ClustalW2 program and box shaded. Dashes (–) indicate gaps in the alignment. The location of the KIR, ESS region, SH2 domain, and SOCS box are indicated above the alignment. Putative PEST sequences between the SH2 domain and the SOCS box predicted by the epestfind program (http://emboss.bioinformatics.nl/cgi-bin/emboss/epestfind) and present in mammalian and amphibian CISH were missing in fish and bird CISH.