Estrogen Regulates MAPK-Related Genes through Genomic and Nongenomic Interactions between IGF-I Receptor Tyrosine Kinase and Estrogen Receptor-Alpha Signaling Pathways in Human Uterine Leiomyoma Cells
Figure 3
Differential expression of phosphorylated (p)IGF-IR, pMAPKp44/42, and ser118 in UtLM cells with scrambled siRNA (siScr) or IGF-IR silencing (siIGF-IR) followed by -estradiol treatment. (a) Western blot of IGF-IR/ER pathway proteins in UtLM cells. (b) Comparison of ratio of densitometric band intensities of phosphorylated (phospho)/total proteins in UtLM cells with siScr or siIGF-IR followed by E2 treatment. Bars represent mean ± SEM of three independent experiments. * versus 0 min.
