Figure 2: Steady-state expression of L-type CaV1.3 channels in the superior region of rat hippocampus. (a) Representative Western blot analysis of proteins extracted from the superior region of hippocampi obtained from postnatal day 1 through 72 rats. Alomone Labs Ltd polyclonal anti-L-type CaV1.3 (ACC-0050) antibodies labeled a high molecular weight band of ~230 kDa and a low molecular weight band of ~200 kDa (top panel). Antibodies against β-actin were used as loading controls for each lane and labeled a band of ~45 kDa (bottom panel). (b) L-type CaV1.3 channel steady-state expression was determined using the integrated optical density (IOD) of the two bands added together, which was divided by the IOD of the band labeled with β-actin antibodies to account for variations in loading. The ratio of IOD/actin for each preparation was further normalized by dividing by the highest ratio from the same film. Data represent mean ± sem ( 𝑁 = 3 or 4).