Figure 7: Immunolabeling of CaV1.3 in hippocampus isolated from rats of different ages. Antibodies against CaV1.3 demonstrated punctate labeling throughout the layers of the CA1 region of the hippocampus across all ages (day 1, 4, 6, 8, 12, 15, 21, and 72). The far left panel is a representative control slide where the primary antibody has been omitted from the incubation. The white bar on this panel represents 20 μm. Each image was background subtracted using the background intensity of its corresponding control slide. For all images the gamma value was maintained at 1.0 and the same peak intensity value for green was utilized for all the images to facilitate direct comparisons of the images.