Mitochondrial Oxidative Stress due to Complex I Dysfunction Promotes Fibroblast Activation and Melanoma Cell Invasiveness
Figure 2
Mutations in NDUFS1 genes (Q522K and R557X/T595A) and in the nuclear PINK gene (W437X) increase fibroblasts migration and invasion.
(a) Boyden cell migration assays of fibroblasts carrying mitochondrial dysfunctions of complex I. 15103 fibroblasts, after 24 hours of serum starvation, were seeded into the upper compartment of Boyden chamber. Cells were allowed to migrate through the filter toward the lower compartment filled with complete medium. Cell invasion was evaluated after Diff-Quick staining by counting cell in six randomly chosen fields. The results are representative of four experiments with similar results
(b) Boyden cell invasion assays of fibroblasts carrying mitochondrial dysfunctions of complex I. Cells were treated as in (a). Cells were allowed to migrate through the filter coated with Matrigel toward the lower compartment filled with complete medium. Cell invasion was evaluated after Diff-Quick staining by counting cell in six randomly chosen fields. The results are representative of four experiments with similar results. * mutated fibroblasts versus control fibroblasts
