Figure 2: Schematic of MAP4K4 and interacting two-hybrid clones. (a) Structure of full length MAP4K4 indicating the N-terminal kinase domain, the C-terminal citron homology domain (CNH), and the location of alternatively spliced modules M1–M9. (b) Structure of the three overlapping clones isolated in the yeast two-hybrid genetic screen which interact with the Pyk2 FERM domain. (c) Structure of the final MAP4K4 clone assembled from SF767 glioma cell isoforms.