956580.fig.003a
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956580.fig.003b
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956580.fig.003c
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956580.fig.003d
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Figure 3: Pyk2 coimmunoprecipitates with MAP4K4. (a) Cells cotransfected with FLAG-epitope-tagged Pyk2 FERM and HA-epitope-tagged MAP4K4(143) were lysed and immunoprecipitated with anti-FLAG antibody or normal mouse IgG. Immunoprecipitates were immunoblotted (IB) with anti-FLAG or anti-HA. WCL: whole cell lysate. (b) Cells were cotransfected with FLAG-epitope-tagged MAP4K4(143) and either HA-tagged Pyk2 or HA-tagged FAK, lysed, and immunoprecipitated with mouse IgG or anti-FLAG antibody. Immunoprecipitates or WCL were immunoblotted as indicated. (c) Cells cotransfected with FLAG-tagged Pyk2 and either HA-tagged MAP4K4(143) or HA-tagged full length (FL) MAP4K4 were lysed and immunoprecipitated with rabbit IgG or anti-HA antibody. Immunoprecipitates or whole cell lysate (WCL) was immunoblotted with the indicated antibodies. (d) SF767 glioma cells were lysed and immunoprecipitated with anti-MAP4K4 antibody. Immunoprecipitates or whole cell lysate (WCL) was immunoblotted with the indicated antibodies.