Journal of Toxicology / 2009 / Article / Fig 2

Research Article

Molecular and Biochemical Effects of a Kola Nut Extract on Androgen Receptor-Mediated Pathways

Figure 2

Stimulation of PSA mRNA expression by Biz-2. LNCaP cells were induced with Biz-2 or DHT, and 5  g of DNase-I-treated RNA isolated from induced or uninduced cells were subjected to two-step Taqman real-time RT-PCR (see Section 2). The relative expression of PSA mRNA expression was calculated by the method. First, relative quantitation of PSA mRNA expression was performed by first normalizing the values of PSA amplification against the values of endogenous 18S rRNA, then the resulting values were normalized using the value of the vehicle control sample. (a) Relative amplification plot of mRNA expression (1–NTC. 2–18S rRNA at 24 hours, 3 and 4- CON at 6 and 24 hours, 5 and 6- Biz-2 at 6 and 24 hours). (b) PSA mRNA expression relative to time. (c) Dose-dependent response of PSA to Biz-2. The values are mean ± SEM of induction samples analyzed in triplicate.