Journal of Toxicology / 2009 / Article / Fig 7

Research Article

Molecular and Biochemical Effects of a Kola Nut Extract on Androgen Receptor-Mediated Pathways

Figure 7

Modulation of apoptotic protein levels in the presence of Biz-2. LNCaP cells were grown in a serum-free medium in the presence of increasing concentrations of Biz-2 for 24 hours. Whole-cell extracts were isolated with RIPA buffer, and 30  g of protein extract were analyzed by immunoblotting for Bcl-2, Bax, AR, and GDPH expression. (a) Immunoblot of Biz-2-induced proteins. (b) Ratio of Bcl2/Bax expression. (c) Biz-2 induces increase in Caspase 3/7 activity.