Shortening and Improving the Embryonic Stem Cell Test through the Use of Gene Biomarkers of Differentiation

Table 1

Primer sequences and annealing temperatures used in the quantitative RT-PCR experiments.

Gene	5^′-3^′ oligo	3^′-5^′ oligo	T (°C)
Actin	CCCTAGGCACCAGGGTGTGA	TCCCAGTTGGTAACAATGCCA	62
Afp	GCTGCAAAGCTGACAACAAG	GGTTGTTGCCTGGAGGTTTC	63
Vegfa	CGTTCACTGTGAGCCTTGTTCAG	GCCTTGCAACGCGAGTCTGT	60
Hdac7	CCATGTTTCTGCCAAATGTTTTGG	GCCGTGAGGTCATGTCCACC	63
Flk1	CAGCCAGACAGACAGTGGGATGGTC	CCGAGGCCACAGACTCCCTGCTT	61
Nes	GCTTTCCTGACCCCAAGCTG	GGCAAGGGGGAAGAGAAGGA	61