Shortening and Improving the Embryonic Stem Cell Test through the Use of Gene Biomarkers of Differentiation

Table 2

The linear discriminant functions and classification criteria considered in EST for assessing the embryotoxicity potential of chemicals. Data taken from [11]. IC₅₀D3 was defined as the concentration that reduces the viability of D3 cells by 50% after 10 days of exposure. IC₅₀3T3 was defined as the concentration that reduces the viability of 3T3 cells by 50% after 10 days of exposure. ID₅₀ was defined as the concentration that inhibits the spontaneous differentiation of D3 embryoid bodies to contractile cardiomyocytes by 50%. IC₅₀D3, IC₅₀3T3, and ID₅₀ must necessarily be expressed in μg/mL.

I = 5.916 log(IC503T3) + 3.500 log(IC50D3) − 5.307[(IC503T3 − ID50)/IC503T3] − 15.27
II = 3.651 log(IC503T3) + 2.394 log(IC50D3) − 2.033[(IC503T3 − ID50)/IC50 3T3] − 6.85
III = −0.125 log(IC503T3) − 1.917 log(IC50D3) + 1.500[(IC503T3 − ID50)/IC503T3] − 2.67
Strong embryotoxic if	Weak embryotoxic if	Non embryotoxic if
III > I and III > II	II > I and II > III	I > II and I > III