Research Article

The Loss of HIF1 Leads to Increased Susceptibility to Cadmium-Chloride-Induced Toxicity in Mouse Embryonic Fibroblasts

Figure 5

Expression and activity of superoxide dismutases. Transcript levels of SOD1 (a) and SOD2 (b) were determined using qRT-PCR in wild-type (WT, white bars) and HIF1α  −/− cells (black bars). Each value was normalized to the wild-type control levels. Cells were left untreated (Ctrl) or treated with 5 μM CdCl2 (Cd2+) for 24 hours. (c) Protein levels of both SOD1 (middle panel) and SOD2 (bottom panel) were determined by Western blotting in wild-type (WT) and HIF1α  −/− cells. β-actin levels (upper panel) were used as a loading control. Cells were treated as in (a) and (b). (d) Total superoxide dismutase enzyme activity in wild-type (WT, white bars) and HIF1α  −/− (black bars) cells were determined. Cells were treated as in (a) and (b). #significant compared to wild-type cells, , .
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