Domoic Acid-Induced Neurotoxicity Is Mainly Mediated by the AMPA/KA Receptor: Comparison between Immature and Mature Primary Cultures of Neurons and Glial Cells from Rat Cerebellum
Figure 5
Changes in the mRNA levels of neural receptors in primary cultures of CGCs exposed to 20 μM domoic acid alone or in combination with antagonists for the AMPA-R (NBQX, 20 μM), NMDA-R (APV, 100 μM), or the neurotransmitter GABA (10 μM) at 1 DIV (immature cultures) or at 7 DIV (mature cultures) for 3 or 10 days. Note that NBQX completely and APV and GABA partly prevent the domoic acid induced downregulation of (a) NMDA-R in immature cultures, (b) NMDA-R in mature cultures, (c) -R mRNA expression in immature cultures, and of (d) -R in mature cultures. Gene expression levels were normalized to the standard calibrator, the housekeeping gene 18S rRNA and the mRNA expression at 1 DIV. Data represent mean ± S.E.M. of three independent experiments performed in duplicates. * ** *** comparing treated to control (untreated) cultures.
