Protective Effects of Liposomal N-Acetylcysteine against Paraquat-Induced Cytotoxicity and Gene Expression
Figure 1
Effect of NAC on the cell viability (a) and uptake of NAC (b) in A549 cells. The viability of cells treated for 24 h with increasing concentrations of NAC was assessed using the MTT assay. Bars represent mean ± S.E.M. of 3 independent experiments performed in octuplet. *denotes significant difference relative to control . For the uptake studies (b), cells were treated with either 5.0 mM NAC- or L-NAC-containing media for various time-points up to 24 h; intracellular NAC levels were measured with a UPLC method as described in Section 2. (solid line: NAC treatment; dotted line: L-NAC treatment). Data points represent mean ± S.E.M. of 3 independent experiments performed in duplicate. †denotes significant difference relative to NAC-treated group .
