Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage Alcoholic Liver Disease

Figure 1

Chronic ethanol ingestion leads to increased hepatic lipid accumulation and lipid peroxidation. (a)   H&E staining reveals marked pan-lobular lipid accumulation following 6 weeks of ethanol consumption. (b) Liver triglycerides are significantly increased following 6 weeks of ethanol ingestion. (c) Immunohistochemical analysis with 4-HNE antibodies reveals increased staining in ethanol-fed mice throughout zones 1 and 2 as early as week 3. (d) Hepatic TBARSs are significantly elevated following 6 weeks of ethanol ingestion. Magnification: 400x. Scale bar represents approximately 50 μm. PT: portal triad; CV: central vein (𝑛=12, *𝑃<0.05, **𝑃<0.01).

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