Safety Assessment of Zigbir®: A Polyherbal Formulation in Sprague-Dawley Rats

Table 5

Clinical chemistry data (mean ± SD) of female rats after oral administration of Zigbir® for 90 days.

Parameters	Control	250 mg/kg	500 mg/kg	1000 mg/kg	Control recovery	1000 mg/kg recovery
Total protein (g%)	7.54 ± 0.38	7.54 ± 0.56	7.83 ± 0.53	7.55 ± 0.46	7.79 ± 0.35	7.64 ± 0.29
Albumin (g%)	3.40 ± 0.32	3.46 ± 0.43	3.18 ± 0.41	3.34 ± 0.48	3.29 ± 0.35	3.67 ± 0.25
Bilirubin (mg%)	0.68 ± 0.07	0.67 ± 0.10	0.73 ± 0.04	0.64 ± 0.08	0.64 ± 0.09	0.66 ± 0.06
Blood sugar (mg%)	92.10 ± 12.48	88.80 ± 13.95	93.80 ± 11.48	83.60 ± 8.57	91.80 ± 10.89	91.00 ± 7.28
Triglycerides (mg%)	104.70 ± 9.14	105.30 ± 9.04	102.50 ± 9.42	106.30 ± 7.79	102.40 ± 3.78	105.60 ± 3.21
Cholesterol (mg%)	63.20 ± 4.47	61.80 ± 6.61	64.50 ± 5.48	64.20 ± 4.98	66.20 ± 3.03	65.40 ± 5.94
BUN (mg%)	38.90 ± 4.82	41.60 ± 4.58	38.30 ± 4.97	36.80 ± 5.71	38.40 ± 7.37	41.40 ± 4.28
Creatinine (mg%)	0.98 ± 0.09	0.97 ± 0.12	0.98 ± 0.11	0.95 ± 0.07	0.97 ± 0.05	1.03 ± 0.10
AST(IU/L)	59.90 ± 5.51	61.20 ± 5.03	62.50 ± 4.55	63.50 ± 4.35	62.20 ± 8.32	65.80 ± 5.97
ALT (IU/L)	43.20 ± 6.36	42.40 ± 7.44	38.90 ± 7.99	39.50 ± 9.44	39.60 ± 6.80	37.60 ± 7.27
ALP (IU/L)	63.90 ± 7.06	66.90 ± 5.34	69.60 ± 8.28	108.80** ± 9.41	71.00 ± 9.08	71.60 ± 6.31
γGT(U/L)	15.50 ± 2.92	14.80 ± 4.32	15.90 ± 2.81	15.90 ± 3.54	15.80 ± 2.39	15.60 ± 3.51
LDH (IU/L)	358.50 ± 33.58	354.50 ± 31.02	371.50 ±16.57	359.60 ± 34.88	334.60 ± 26.69	369.80 ± 34.49
CPK (IU/L)	63.70 ± 4.22	63.20 ± 5.01	64.50 ± 4.50	62.60 ± 4.79	66.40 ± 3.13	65.00 ± 5.66
Sodium (mmol/L)	142.10 ± 5.30	138.80 ± 5.69	141.80 ± 6.20	139.70 ± 6.31	139.40 ± 3.36	136.00 ± 5.10
Chloride (mmol/L)	101.90 ± 3.51	101.80 ± 2.82	101.40 ± 3.63	101.20 ± 2.94	102.40 ± 1.67	102.20 ± 1.79
Potassium (mmol/L)	3.73 ± 0.15	3.51* ± 0.14	3.83 ± 0.15	3.68 ± 0.24	3.77 ± 0.08	3.79 ± 0.06
Calcium (mg%)	9.44 ± 0.60	9.41 ± 0.50	9.49 ± 0.67	9.47 ± 0.70	9.52 ± 0.50	9.98 ± 0.68
Phosphorus (mg%)	4.23 ± 0.31	4.11 ± 0.27	4.21 ± 0.39	4.30 ± 0.37	4.34 ± 0.44	4.00 ± 0.50

∗P < 0.05 versus control group.
∗∗P < 0.01 versus control group.