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Journal of Toxicology
Volume 2013, Article ID 161496, 12 pages
http://dx.doi.org/10.1155/2013/161496
Research Article

Acetaldehyde Content and Oxidative Stress in the Deleterious Effects of Alcohol Drinking on Rat Uterine Horn

1Centro de Investigaciones Toxicológicas (CEITOX-UNIDEF, MINDEF-CONICET), Juan B de La Salle 4397, B1603ALO Villa Martelli, Argentina
2Instituto de Investigación e Ingeniería Ambiental, Universidad Nacional de General San Martín, Avenue 25 de Mayo y Francia, 1650 San Martín, Argentina

Received 8 July 2013; Revised 10 September 2013; Accepted 16 September 2013

Academic Editor: Robert Tanguay

Copyright © 2013 Lara Romina Buthet et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

After alcohol exposure through a standard Lieber and De Carli diet for 28 days, a severe atrophy in the rat uteirne horn was observed, accompanied by significant alterations in its epithelial cells. Microsomal pathway of acetaldehyde production was slightly increased. Hydroxyl radicals were detected in the cytosolic fraction, and this was attributed to participation of xanthine oxidoreductase. They were also observed in the microsomal fraction in the presence of NADPH generating system. No generation of 1-hydroxyethyl was evidenced. The t-butylhydroperoxide-induced chemiluminescence analysis of uterine horn homogenates revealed a significant increase in the chemiluminiscence emission due to ethanol exposure. In the animals repeatedly exposed to alcohol, sulfhydryl content from uterine horn proteins was decreased, but no significant changes were observed in the protein carbonyl content from the same samples. Minor but significant decreasing changes were observed in the GSH content accompanied by a tendency to decrease in the GSH/GSSG ratio. A highly significant finding was the diminished activity content of glutathione peroxidase. Results suggest that acetaldehyde accumulation plus the oxidative stress may play an additional effect to the alcohol-promoted hormonal changes in the uterus reported by others after chronic exposure to alcohol.