Acetaldehyde Content and Oxidative Stress in the Deleterious Effects of Alcohol Drinking on Rat Uterine Horn

Table 4

Protein sulfhydryl and protein carbonyl in uterine horn from rats receiving an alcohol containing liquid diet.

	nmol CO/mg protein^a	nmol SH/mg protein^b
Control	7.12±1.16	11.32±0.78
EtOH-treated	7.65±1.08	9.71±0.66

aUterine horn tissue isolated from Control and EtOH-treated rats was homogenized in 0.15 M Tris-HCl/1 mM KH₂PO₄ (pH 7.4) and centrifuged at 600 ×g, and the supernatants were used to carbonyl determination as described in Section 2. Three samples per group were run, each consisting of a homogenate from a separate lot of pooled uterine horn tissue (five animals each). 𝑃>0.05 (Control versus EtOH-treated).
^bUterine horn tissue isolated from Control and EtOH-treated rats was homogenized in 0.15 M Tris-HCl/1 mM KH₂PO₄ (pH 7.4) and centrifuged at 600 ×g and the supernatants were used to sulfhydryl determination as described in Section 2. Three samples per group were run, each consisting of a homogenate from a separate lot of pooled uterine horn tissue (five animals each). 𝑃<0.05 (Control versus EtOH-treated).