Research Article

The Aryl-Hydrocarbon Receptor Protein Interaction Network (AHR-PIN) as Identified by Tandem Affinity Purification (TAP) and Mass Spectrometry

Figure 7

TCDD treatment influences Mrpl40 mitochondrial localization. (a) Cellular fractionation was performed on AHR in wild type Hepa1c1c7 cells. Fractions from cells exposed to TCDD for 6 hours were probed with antibodies specific to MRPL40, lactate dehydrogenase (LDH), and cytochrome c oxidase subunit 4 (COXIV). The results demonstrate a decreased level of mitochondrial localized Mrpl40 following TCDD exposure. (b) Similar cellular fractionations were next performed on AHR null Hepac12 cells. No decrease in Mrpl40 was observed after exposure to TCDD for 6 hours. LDH was used as the cytosolic fraction control protein and COXIV was used as the purified mitochondrial fraction control protein.