Differential Effects of Methyl-4-Phenylpyridinium Ion, Rotenone, and Paraquat on Differentiated SH-SY5Y Cells
Figure 3
ROT-induced neurotoxicity showed a biphasic pattern with mitochondrial dysfunction and cell death in differentiated SH-SY5Y cells. The cells were incubated with 1, 10, and 100 μM of ROT, and then MTT and LDH tests were performed at 24, 48, and 72 h time points. (a) Increase in LDH release in units per liter (U/L) into the medium in differentiated SH-SY5Y cells. The delta increase in LDH release into the medium was calculated by subtraction of the respective controls (results were pooled from 3 different experiments, each experiment having 6 different culture wells per condition). (b) Mitochondrial dysfunction in differentiated SH-SY5Y cells evaluated by the MTT test. The viability of untreated control cells was set to 100%, and all treatments were expressed as the percentage of the respective controls (results were pooled from 3 different experiments, each experiment having 6 different culture wells per condition). The means were compared using the two-way ANOVA, followed by the Bonferroni post hoc test (*** concentration versus control; ##, ### concentration versus concentration).
