Research Article

The Effects of Eucheuma cottonii on Signaling Pathway Inducing Mucin Synthesis in Rat Lungs Chronically Exposed to Particulate Matter 10 (PM10) Coal Dust

Figure 7

Representative immunofluorescence with anti-EGFR and anti-MUC5AC antibodies for determination of the lung EGFR and MUC5AC expressions in rats. These expressions were analyzed by counting fluorescent intensity of cells (in arbitrary units (AU)) in five random high-power microscope fields. The fluorescent images were recorded under a confocal laser scanning microscope. Cells were shown EGFR positive (red fluorescent) and MUC5AC positive (green fluorescent). Differential interference contrast (DIC); non-exposure group (N); group exposed to coal dust at dose of 25 mg/m3 (CD25); group supplemented with the ethanolic extract of E. cottonii at dose of 300 mg/kg BW (EC300).
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