Epigenetically Mediated Pathogenic Effects of Phenanthrene on Regulatory T Cells

Figure 3

Phenanthrene exposure results in Treg to Teff conversion. Isolated Treg from individual healthy donors (𝑛=5) were incubated with 300 nM Phe for 0 to 7 days and analyzed for T cell phenotype. Cells were analyzed at 5 time points by immunophenotyping for Treg (CD4⁺CD25^hiCD127^lo, gray bars) and Teff (CD4⁺CD25^neg/lo, black bars) subsets in cultures exposed to Phe (a) versus diluent alone (b). Teff incubated with Phe did not show decreases in cell number over time (c). Error bars show SD.

(a)

(b)

(c)