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Journal of Toxicology
Volume 2013, Article ID 967029, 13 pages
Research Article

Epigenetically Mediated Pathogenic Effects of Phenanthrene on Regulatory T Cells

Stanford University School of Medicine, Division of Immunology and Allergy, Grant Building, 3rd Floor, S370, MC5208, Stanford, CA 94305, USA

Received 21 October 2012; Revised 4 January 2013; Accepted 7 January 2013

Academic Editor: Maria Teresa Colomina

Copyright © 2013 Jing Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

T Cell Isolation and Phenotyping Healthy donor samples were obtained from the Stanford Blood Center and sorting via flow cytometry was performed (FACS Aria, BD Biosciences). Each population obtained from flow cytometry was tested for purity based on multicolor flow cytometry staining for CD4+CD25hiCD127lo (Treg) and CD4+CD25neg (conventional CD4+ T cell). Only samples meeting or exceeding 97% pure were used for further experimentation and analysis. Fluorescently labeled, directly conjugated antibodies were used. Further descriptions are provided in the supplementary material.

  1. Supplementary Material