High Content Imaging and Analysis Enable Quantitative In Situ Assessment of CYP3A4 Using Cryopreserved Differentiated HepaRG Cells

Figure 8

Quantification of CYP3A4 expression changes via HCA. Using quantitative HCA image analysis, the cellular intensity of CYP3A4 staining in small nuclei HepaRG cells treated with vehicle or 10 μM rifampicin at each cell seeding density was determined. Data represent mean ± SEM for 3 independent experiments. represents versus DMSO vehicle controls at the same cell seeding density; represents versus HepaRG cells seeded at 25,000 cells per well.