Proliferation and 1/2 Cytokine Production in Human Peripheral Blood Mononuclear Cells after Treatment with Cypermethrin and Mancozeb In Vitro

Table 1

Effect on lymphocyte proliferation (%) and / cytokine production (pg/mL) in PBMC cultures. Values shown are average ± SD from three volunteers (each was conducted in triplicate).

(a)

Mancozeb (M)	Proliferation	TNF-	IFN-	IL-2	IL-10	IL-6	IL-4
Solv.	100.0 ± 0.0	696.4 ± 60.2	1303.4 ± 175.1	56.6 ± 10.9	37.7 ± 7.2	597.8 ± 63.6	19.7 ± 3.1
0.45	105.4 ± 2.5	657.4 ± 69.3	1912.3 ± 127.6	109.4 ± 12.9	41.0 ± 7.3	628.8 ± 69.1	22.2 ± 4.9
0.9	119.9 ± 4.5	621.1 ± 66.9	2401.0 ± 113.0	138.1 ± 23.5	39.8 ± 8.3	551.2 ± 86.1	19.6 ± 4.6
1.87	138.4 ± 6.8	424.7 ± 21.7	2617.8 ± 165.6	152.9 ± 22.2	33.6 ± 8.7	390.6 ± 100.4	17.4 ± 3.1
3.75	162.5 ± 4.6	238.6 ± 35.8	2974.6 ± 411.6	163.7 ± 26.6	24.2 ± 9.6	217.5 ± 72.2	14.5 ± 2.7
7.5	174.5 ± 5.2	129.1 ± 25.2	3539.2 ± 103.7	167.3 ± 28.6	15.1 ± 4.6	107.7 ± 34.9	10.0 ± 1.7

(b)

Cypermethrin (M)	Proliferation	TNF-	IFN-	IL-2	IL-10	IL-6	IL-4
Solv.	100.0 ± 0.0	426.6 ± 44.4	2555.9 ± 281.0	127.4 ± 6.2	31.9 ± 5.8	614.5 ± 90.8	17.9 ± 3.2
1.87	102.7 ± 1.4	412.0 ± 22.8	2450.4 ± 352.0	138.4 ± 8.9	28.9 ± 4.9	629.0 ± 31.0	16.2 ± 2.8
3.75	102.7 ± 3.4	409.6 ± 54.3	2421.8 ± 244.5	148.6 ± 9.8	25.9 ± 4.4	601.2 ± 27.0	15.3 ± 2.5
7.5	101.1 ± 1.2	397.8 ± 59.0	2316.2 ± 310.4	145.3 ± 7.9	25.9 ± 2.8	565.9 ± 30.4	13.6 ± 1.8
15	100.0 ± 1.1	382.4 ± 57.0	2222.3 ± 336.4	149.6 ± 13.4	24.3 ± 3.2	538.2 ± 33.9	13.0 ± 1.4
30	98.0 ± 0.8	365.1 ± 42.5	2134.9 ± 364.2	159.6 ± 12.8	23.0 ± 3.3	527.3 ± 34.1	12.1 ± 1.2